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Influence of Mercury Exposure on Oxidative Stress Biomarkers in Longitudinal Muscle of Holothuria forskali

Received: 20 November 2020     Accepted: 9 December 2020     Published: 16 December 2020
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Abstract

Mercury (Hg) pollution is featuring as one of the major threat for marine ecosystem, biota and human health. Thereby, the objective of the current study was to evaluate the effect of environmentally relevant concentrations of mercury on oxidative stress, enzymatic and non-enzymatic antioxidant defenses and metallothionein levels in the longitudinal muscle of sea cucumber Holothuria forskali. With this purpose, sea cucumber were exposed to graded concentrations of mercury chloride HgCl2 (40, 80 and 160 µg L-1) for 96 h under controlled conditions. Our findings revealed that Hg burden in the longitudinal muscle tended to increase with increasing HgCl2 concentrations. The Hg exposure promoted muscular oxidative stress as evidenced by the increased levels of malondialdehyde (MDA), advanced oxidation protein product (AOPP) and metallothionein (MT) of treated animals compared with controls. Additionally, significant increases in the activities of the enzymatic (glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT)) and non-enzymatic (non-protein thiol (NPSH) and glutathione (GSH)) antioxidants were also observed in all treated groups. Overall, this study proved that mercury is able to produce deleterious effects even at the lowest environmentally-realistic concentration in the Holothuria forskali longitudinal muscle which may be considered as a target tissue of mercury accumulation in holothurian.

Published in International Journal of Ecotoxicology and Ecobiology (Volume 5, Issue 4)
DOI 10.11648/j.ijee.20200504.13
Page(s) 54-60
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Holothuria forskali, Mercuric Chloride (HgCl2), Longitudinal Muscle Hg Burden, Enzymatic and Non-enzymatic Antioxidant

References
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  • APA Style

    Imen Rabeh, Khaoula Telahigue, Tarek Hajji, Chaima Fouzai, Safa Bejaoui, et al. (2020). Influence of Mercury Exposure on Oxidative Stress Biomarkers in Longitudinal Muscle of Holothuria forskali. International Journal of Ecotoxicology and Ecobiology, 5(4), 54-60. https://doi.org/10.11648/j.ijee.20200504.13

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    ACS Style

    Imen Rabeh; Khaoula Telahigue; Tarek Hajji; Chaima Fouzai; Safa Bejaoui, et al. Influence of Mercury Exposure on Oxidative Stress Biomarkers in Longitudinal Muscle of Holothuria forskali. Int. J. Ecotoxicol. Ecobiol. 2020, 5(4), 54-60. doi: 10.11648/j.ijee.20200504.13

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    AMA Style

    Imen Rabeh, Khaoula Telahigue, Tarek Hajji, Chaima Fouzai, Safa Bejaoui, et al. Influence of Mercury Exposure on Oxidative Stress Biomarkers in Longitudinal Muscle of Holothuria forskali. Int J Ecotoxicol Ecobiol. 2020;5(4):54-60. doi: 10.11648/j.ijee.20200504.13

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  • @article{10.11648/j.ijee.20200504.13,
      author = {Imen Rabeh and Khaoula Telahigue and Tarek Hajji and Chaima Fouzai and Safa Bejaoui and Lassaad Chouba and Mhamed El Cafsi and Nejla Soudani},
      title = {Influence of Mercury Exposure on Oxidative Stress Biomarkers in Longitudinal Muscle of Holothuria forskali},
      journal = {International Journal of Ecotoxicology and Ecobiology},
      volume = {5},
      number = {4},
      pages = {54-60},
      doi = {10.11648/j.ijee.20200504.13},
      url = {https://doi.org/10.11648/j.ijee.20200504.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijee.20200504.13},
      abstract = {Mercury (Hg) pollution is featuring as one of the major threat for marine ecosystem, biota and human health. Thereby, the objective of the current study was to evaluate the effect of environmentally relevant concentrations of mercury on oxidative stress, enzymatic and non-enzymatic antioxidant defenses and metallothionein levels in the longitudinal muscle of sea cucumber Holothuria forskali. With this purpose, sea cucumber were exposed to graded concentrations of mercury chloride HgCl2 (40, 80 and 160 µg L-1) for 96 h under controlled conditions. Our findings revealed that Hg burden in the longitudinal muscle tended to increase with increasing HgCl2 concentrations. The Hg exposure promoted muscular oxidative stress as evidenced by the increased levels of malondialdehyde (MDA), advanced oxidation protein product (AOPP) and metallothionein (MT) of treated animals compared with controls. Additionally, significant increases in the activities of the enzymatic (glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT)) and non-enzymatic (non-protein thiol (NPSH) and glutathione (GSH)) antioxidants were also observed in all treated groups. Overall, this study proved that mercury is able to produce deleterious effects even at the lowest environmentally-realistic concentration in the Holothuria forskali longitudinal muscle which may be considered as a target tissue of mercury accumulation in holothurian.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Influence of Mercury Exposure on Oxidative Stress Biomarkers in Longitudinal Muscle of Holothuria forskali
    AU  - Imen Rabeh
    AU  - Khaoula Telahigue
    AU  - Tarek Hajji
    AU  - Chaima Fouzai
    AU  - Safa Bejaoui
    AU  - Lassaad Chouba
    AU  - Mhamed El Cafsi
    AU  - Nejla Soudani
    Y1  - 2020/12/16
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ijee.20200504.13
    DO  - 10.11648/j.ijee.20200504.13
    T2  - International Journal of Ecotoxicology and Ecobiology
    JF  - International Journal of Ecotoxicology and Ecobiology
    JO  - International Journal of Ecotoxicology and Ecobiology
    SP  - 54
    EP  - 60
    PB  - Science Publishing Group
    SN  - 2575-1735
    UR  - https://doi.org/10.11648/j.ijee.20200504.13
    AB  - Mercury (Hg) pollution is featuring as one of the major threat for marine ecosystem, biota and human health. Thereby, the objective of the current study was to evaluate the effect of environmentally relevant concentrations of mercury on oxidative stress, enzymatic and non-enzymatic antioxidant defenses and metallothionein levels in the longitudinal muscle of sea cucumber Holothuria forskali. With this purpose, sea cucumber were exposed to graded concentrations of mercury chloride HgCl2 (40, 80 and 160 µg L-1) for 96 h under controlled conditions. Our findings revealed that Hg burden in the longitudinal muscle tended to increase with increasing HgCl2 concentrations. The Hg exposure promoted muscular oxidative stress as evidenced by the increased levels of malondialdehyde (MDA), advanced oxidation protein product (AOPP) and metallothionein (MT) of treated animals compared with controls. Additionally, significant increases in the activities of the enzymatic (glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT)) and non-enzymatic (non-protein thiol (NPSH) and glutathione (GSH)) antioxidants were also observed in all treated groups. Overall, this study proved that mercury is able to produce deleterious effects even at the lowest environmentally-realistic concentration in the Holothuria forskali longitudinal muscle which may be considered as a target tissue of mercury accumulation in holothurian.
    VL  - 5
    IS  - 4
    ER  - 

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Author Information
  • Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

  • Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

  • Higher Institute of Biotechnology - Sidi Thabet, Biotechpole Sidi Thabet, Univ. Manouba, Ariana, Tunisia

  • Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

  • Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

  • National Institute of Marine Science and Technology (INSTM), La Goulette, Tunis, Tunisia

  • Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

  • Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

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