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Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile

Received: 24 May 2016     Accepted: 12 July 2016     Published: 26 August 2016
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Abstract

Genetic population analyses for Nile fishes are very scarce. Nile pufferfish Tetraodon lineatus (Linnaeus, 1758), is a widely-distributed freshwater fish, with no known major widespread threats. It has been classed as 'Endangered'. Little is known concerning its biology and genetics in Egypt. Hence, this work was designed to study the genetic diversity and conservation status of T. lineatus for the first time in Egypt and Africa. DNA barcoding was carried out through PCR-amplification and sequencing of the cytochrome c oxidase gene barcode 5´ region of forty-five samples obtained from three different localities in Upper Egypt. Only three haplotypes could be characterized in all samples. The other population analyses showed clear population loss of extension and potential bottleneck, what may explain the severe drop of species records in the Northern areas of the Nile. Phylogenetic analysis exhibited the monophyletic origin T. lineatus and other African freshwater pufferfishes, more probably as descendants from an Indo-West Pacific ancestor. We highly recommend the fulfilling of more studies concerning the biology, ecology, and genetics of the species as major steps towards its proper conservation and understanding of its adaptation to different natural and man-made constraints in the River Nile system.

Published in International Journal of Ecotoxicology and Ecobiology (Volume 1, Issue 2)
DOI 10.11648/j.ijee.20160102.15
Page(s) 60-66
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Africa, COI, PCR, Population Genetics, Pufferfish, Tetraodon lineatus

References
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Cite This Article
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    Khaled Mohammed-Geba, Aldoushy A. Mahdy, Ahmed S. A. Eissa, Alaa G. M. Osman. (2016). Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile. International Journal of Ecotoxicology and Ecobiology, 1(2), 60-66. https://doi.org/10.11648/j.ijee.20160102.15

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    ACS Style

    Khaled Mohammed-Geba; Aldoushy A. Mahdy; Ahmed S. A. Eissa; Alaa G. M. Osman. Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile. Int. J. Ecotoxicol. Ecobiol. 2016, 1(2), 60-66. doi: 10.11648/j.ijee.20160102.15

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    AMA Style

    Khaled Mohammed-Geba, Aldoushy A. Mahdy, Ahmed S. A. Eissa, Alaa G. M. Osman. Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile. Int J Ecotoxicol Ecobiol. 2016;1(2):60-66. doi: 10.11648/j.ijee.20160102.15

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  • @article{10.11648/j.ijee.20160102.15,
      author = {Khaled Mohammed-Geba and Aldoushy A. Mahdy and Ahmed S. A. Eissa and Alaa G. M. Osman},
      title = {Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile},
      journal = {International Journal of Ecotoxicology and Ecobiology},
      volume = {1},
      number = {2},
      pages = {60-66},
      doi = {10.11648/j.ijee.20160102.15},
      url = {https://doi.org/10.11648/j.ijee.20160102.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijee.20160102.15},
      abstract = {Genetic population analyses for Nile fishes are very scarce. Nile pufferfish Tetraodon lineatus (Linnaeus, 1758), is a widely-distributed freshwater fish, with no known major widespread threats. It has been classed as 'Endangered'. Little is known concerning its biology and genetics in Egypt. Hence, this work was designed to study the genetic diversity and conservation status of T. lineatus for the first time in Egypt and Africa. DNA barcoding was carried out through PCR-amplification and sequencing of the cytochrome c oxidase gene barcode 5´ region of forty-five samples obtained from three different localities in Upper Egypt. Only three haplotypes could be characterized in all samples. The other population analyses showed clear population loss of extension and potential bottleneck, what may explain the severe drop of species records in the Northern areas of the Nile. Phylogenetic analysis exhibited the monophyletic origin T. lineatus and other African freshwater pufferfishes, more probably as descendants from an Indo-West Pacific ancestor. We highly recommend the fulfilling of more studies concerning the biology, ecology, and genetics of the species as major steps towards its proper conservation and understanding of its adaptation to different natural and man-made constraints in the River Nile system.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile
    AU  - Khaled Mohammed-Geba
    AU  - Aldoushy A. Mahdy
    AU  - Ahmed S. A. Eissa
    AU  - Alaa G. M. Osman
    Y1  - 2016/08/26
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ijee.20160102.15
    DO  - 10.11648/j.ijee.20160102.15
    T2  - International Journal of Ecotoxicology and Ecobiology
    JF  - International Journal of Ecotoxicology and Ecobiology
    JO  - International Journal of Ecotoxicology and Ecobiology
    SP  - 60
    EP  - 66
    PB  - Science Publishing Group
    SN  - 2575-1735
    UR  - https://doi.org/10.11648/j.ijee.20160102.15
    AB  - Genetic population analyses for Nile fishes are very scarce. Nile pufferfish Tetraodon lineatus (Linnaeus, 1758), is a widely-distributed freshwater fish, with no known major widespread threats. It has been classed as 'Endangered'. Little is known concerning its biology and genetics in Egypt. Hence, this work was designed to study the genetic diversity and conservation status of T. lineatus for the first time in Egypt and Africa. DNA barcoding was carried out through PCR-amplification and sequencing of the cytochrome c oxidase gene barcode 5´ region of forty-five samples obtained from three different localities in Upper Egypt. Only three haplotypes could be characterized in all samples. The other population analyses showed clear population loss of extension and potential bottleneck, what may explain the severe drop of species records in the Northern areas of the Nile. Phylogenetic analysis exhibited the monophyletic origin T. lineatus and other African freshwater pufferfishes, more probably as descendants from an Indo-West Pacific ancestor. We highly recommend the fulfilling of more studies concerning the biology, ecology, and genetics of the species as major steps towards its proper conservation and understanding of its adaptation to different natural and man-made constraints in the River Nile system.
    VL  - 1
    IS  - 2
    ER  - 

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Author Information
  • Department of Zoology, Faculty of Science, Menoufia University, 32511 Shebin El-Kom, Egypt

  • Department of Zoology, Faculty of Science, Al-Azhar University, Assiut branch, 71524 Assiut, Egypt

  • Department of Fisheries, Organization of World Fish, Aswan, Egypt

  • Department of Zoology, Faculty of Science, Al-Azhar University, Assiut branch, 71524 Assiut, Egypt

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